To further explore the efficacy of Ace-031, a famous pharmaceutical company did a research in 2007. At that point the company only had animal studies to back them up. Several years later, with the continuous improvement of science and technology, they published the results of a human study in 2013, in which 48 healthy women aged from 45-75 had been given a single injection containing 0.02, 0.05, 0.1, 0.3, 1 or 3 mg ACE-031 per kg bodyweight. The substance remained in circulation in the body for a number of weeks: the half-life was 10-15 days.

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