BPC-157 and TB-500

What is BPC-157

Sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val.

Molecular Formula: C62H98N16O22

Molecular Weight: 1419.556 g/mol


Chemical Names: Bpc 15; Bpc 157; Booly protection compound 15; Bpc-157; BPC-15; UNII-8ED8NXK95P

BPC 157 (body protection compound -157) is a 15-amino acid pentapeptide.The amino acid sequence in BPC157 is similar to that in human BPC.Human stomach BPC exists in gastric juice.Experiments showed that BPC 157 enhanced wound healing, including tendon wounds, such as the transected Achilles tendon in rats.The aim of this study was to investigate the possible mechanisms by which BPC 157 May be used to accelerate the healing process of injured tendons.The study used two groups of tendon explants, one cultured in a medium containing BPC 157 and the other without BPC 157.The cultures were then examined for tendon fibroblast growth.This growth indicates tendon regeneration.The results showed that compared with the culture lacking BPC 157, the growth of foreign and domestic explants in the culture containing BPC 157 was significantly accelerated.In addition, MTT assays did show that BPC 157 did not directly affect cell proliferation.Rat Achilles tendon culture.However, the results also showed that BPC 157 significantly increased cell survival under oxidative stress.In addition, Transwell filter migration assays showed that BPC 157 significantly increased fibroblast migration in vitro in a dose-dependent manner.In addition, BPC 157 accelerated the dispersion of fibroblasts in culture dishes in a dose-dependent manner.

In addition, fitc-ghostpen cyclic peptide staining demonstrated that BPC157 induced the formation of f-actin in fibroblasts.Similarly, Western blot analysis can detect the production and activation of pilin and FAK protein.Western blot analysis also showed that BPC 157 increased the phosphorylation of pilin and FAK without affecting the amount produced.

Therefore, it was concluded that BPC 157 enhanced in vitro growth and migration of fibroblasts derived from rat tendon explants.In addition, BPC 157 increased the possibility of cell survival under oxidative stress.These effects of BPC157 may be mediated by activation (via phosphorylation) of the fak-pilin pathway of the protein.

What is TB-500

Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser

Molecular formula: C212H350N56O78S

Molar Mass: 4963.4408

CAS number: 77591-33-4

Synonyms: Thymosin Beta 4

Thymosin Beta 4 or tb-500 is a synthetic form of the naturally occurring 43-amino acid peptide found in almost all human and animal cells studied.

A 2010 study in the proceedings of the New York academy of sciences supports the TB500’s potential to repair heart muscle after injury, such as after a heart attack.Recognizing the limitations of stem cell therapy in this application, it was found that TB500 inhibited myocardial cell death, stimulated vascular growth, and activated cardiac processes that promote healing after injury.Investigations have shown that TB500 may be the first dose that can voluntarily restore damaged heart muscle after a heart attack.This was further confirmed by previous mouse studies in 2004, showing migration, survival, and repair of myocardial injury.

Filamentous actin (F- actin or actin) forms a polymer that thickens sputum and adversely affects patients with cystic fibrosis.TB500 has been studied in CF patient populations, and the results show that the cohesion of sputum decreases in dose and time after the use of TB500 in combination with dornase alfa.Combined treatment showed a 71% improvement in mucosal ciliary transport and a 44% improvement in mucous cough transport.

TB500 is known to stimulate myoblasts and muscle cells (muscle-producing cells).Mitochondrial RNA levels in TB500 have been shown to increase after muscle injury, helping to regenerate muscle fibers and resolve inflammation at the site of injury.This data supports that muscle injury leads to increased local production of TB500 and promotes the migration of incoming myoblasts to accelerate skeletal muscle regeneration.

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